• Título:  RAPID TEST FOR DETECTION OF BACTERIA AND BIOFILMS
• Autor:  Bazsefidpar bazsefidpar, Shayesteh
• Universidad:  Universidad de Oviedo
• Departamento:  Química física y analítica
• Fecha de Lectura:  31/10/2023
• Mención Europeo / Mención Internacional: concedido
• Programa de doctorado:  Programa de Doctorado en Análisis Químico, Bioquímico y Estructural y Modelización Computacional por la Universidad de Oviedo
• Dirección: 
  • BLANCO LÓPEZ, CARMEN (DIRECTOR)
  • MATOS GONZÁLEZ, MARÍA (CODIRECTOR)
• Tribunal: 
  • LOBO CASTAÑON, MARIA JESUS (PRESIDENTE)
  • HEVIA SANCHEZ, DAVID (SECRETARIO)
  • Caserta, Sergio (VOCAL)
  • MARCO COLAS, MARIA DEL PILAR (VOCAL)
  • FERNÁNDEZ GARCIA, MARÍA (VOCAL)
• Descriptores: 
  • BACTERIAS
• Localización:  BIBLIOTECA CENTRAL UNIVERSIDAD DE OVIEDO
• Resumen:
  In recent years, bacterial biofilms have emerged as a significant challenge in various fields, including medicine, industry, and environmental sciences. Biofilms are notorious for their ability to resist antibiotics and other treatments, leading to chronic infections becoming an important clinical challenge. Rapid and sensitive detection of bacterial biofilm formation is essential to prevent biofilm-related complications and to improve treatment outcomes. This thesis aimed to develop a cost-effective and rapid lateral flow immunoassay (LFIA) platform for the early detection of planktonic bacteria of specifically E. coli O157:H7, as a target analyte for biofilm detection. First, state of the art research consisted of a review article on biomolecules involved in biofilm formation, followed by critical analysis of current biosensors for detecting biofilms and recent progress made in LFIA for early biofilm detection. Then, several types of multilabel systems were developed and tested as detection labels with distinct properties, including superparamagnetic iron oxide nanoparticles (SPIONs) encapsulated by lipid-polymer nanoparticles (SPIONs @ PLGA PVA/PC), core-shell Fe3O4@Au magnetic nanoparticles, PEGylated spherical gold nanoparticles (AuNP), gold nanorods (AuNRs) and gold nanoprisms (AuNPrs). Different signal amplification strategies were used, including silver enhancement, and coupling with electrochemical, and thermal transducers. The results demonstrated the potential of the developed LFIA platform in detecting E. coli O157:H7 in the early stages of biofilm formation. Additionally, an alternative approach for rapid planktonic cell detection was the electrochemical magnetic immunoassay utilizing Fe3O4@Au MNPs. This approach offers a cost-effective, portable, and user-friendly solution, eliminating the need for expensive equipment. The thesis suggests that these methods have the potential to be adapted for the detection of other pathogens by using different specific bioreceptors. Overall, this study contributes to the advancement of a more specific, versatile, and reliable LFIA platform for early-stage detection of bacterial biofilms. The findings hold great potential for improving human health, food safety, and medical safety.