Tesis doctorales: TESEO

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Ministerio de Universidades - Gobierno de España

Título: CONTROL OF ENTERIC PARASITIC DISEASES OF FARMED GILTHEAD SEA BREAM: NEW INSIGHTS INTO ENTEROMYXUM LEEI (MYXOZOA) AND ENTEROSPORA NUCLEOPHILA (MICROSPORIDIA) INFECTIONS.
Autor: Picard Sánchez, María Amparo
Universidad: Universitat Politècnica de València
Departamento: Escuela de Doctorado de la Universitat Politècnica de València
Fecha de Lectura: 26/04/2021
Mención Europeo / Mención Internacional: Concedido
Programa de doctorado: Programa de Doctorado en Ciencia y Tecnología de la Producción Animal por la Universitat Politècnica de València
Dirección:
- SITJÀ BOBADILLA, ARIADNA (DIRECTOR)
- Piazzon de Haro, María Carla (DIRECTOR)
- PALENZUELA RUIZ, OSWALDO (DIRECTOR)
- Asturiano Nemesio, Juan Francisco (TUTOR/PONENTE)
Tribunal:
- MONTERO ROYO, FRANCISCO ESTEBAN (PRESIDENTE)
- DE BLAS GIRAL, IGNACIO (SECRETARIO)
- Bartholomew, Jerri L. (VOCAL)
Descriptores:
- PARASITOLOGIA ANIMAL
- INMUNOLOGIA
- PATOLOGIA ANIMAL
Localización: BIBLIOTECA CENTRAL UNIVERSITAT POLITÈCNICA DE VALÈNCIA

http://hdl.handle.net/10251/167035

Marcador: https://www.educacion.gob.es/teseo/mostrarRef.do?ref=465999Añadir a favoritos.
Resumen:
Aquaculture production is hampered by the emergence of parasite diseases in fish farming systems. Among them, in Sparus aurata, there are two important enteric parasites described: Enteromyxum leei (Myxozoa) Enterospora nucleophila (Microsporidia). To date, no in vitro culture has been established for either parasite, and only for E. leei was it possible to establish a model for maintaining the infection in vivo. The aim of this thesis is to gain new knowledge about these parasites and their relationship with the host, also the basic foundations for generating solutions that can be applied in aquaculture. The general objective for E. leei was to study the acquired immunity induced in gilthead bream and the possibility of generating diagnostic tools and vaccines against this disease. To this end, resistance against the parasite was assessed with a second exposure against the parasite, which showed a resistance for at least 16 months. Besides resistance seemed to be correlated with high levels of specific immunoglobulin (Ig) M in blood, and a high expression of Igs, in particular, the soluble forms, even before re-exposure to the parasite. The next step was refining the protocol for effluent infection with E. leei by studying infection at different exposure time points, temperatures and population densities. The results showed that one week of exposure is sufficient to spread E. leei infection by effluent, regardless of temperature. After demonstrating the resistance against E. leei, and with a refined infection model, a passive immunization assay was performed. The results showed that the serum with specific antibodies effectively slows down the invasion of the gut by the parasite and reduces the symptoms of the disease. At the same time, the analysis of the repertoire of the variable regions of intestinal IgM and IgT showed an induction of a polyclonal response in B cells. On the basis of these results, a research was carried out for E. leei antigens that could have use as candidates for the production of vaccines (proteomic study) or diagnostic tools (in silico study) using the parasite transcriptomic data. To do this, a de novo transcriptome was assembled using a mixed sample of gilthead sea bream and parasite, with a posterior filtrate of the sequences. The In silico and proteomic analysis search resulted in 7 and 12 transcripts, respectively, which are being used for diagnostic and vaccine production. The starting point was more basic in E. nucleophila studies, since this is a recently described disease. The samples of this parasite can only be obtained from natural outbreaks in fish farms. Therefore, first study was carried out to characterize the pathology of the infection of naturally infected fish. In the early stages of the infection, the parasite is mainly located in the intestine, but months later, the prevalence is lower in the intestine and increases in the hematopoietic organs and the stomach. Clinical signs of infection were significant reduction in growth, wasting, and intestinal walls paleness. At the cellular level, in the most severe cases hypercellularity in the intestinal epithelium, proliferation of rodlet cells, high number of lymphocytes at the base of the epithelium and infiltration of acidophilic granulocytes in the intestinal epithelium were observed. Finally, horizontal transmission of E. nucleophila was tried using different transmission methods: cohabitation, effluent, and oral and anal intubation. Transmission of the parasite was achieved with all routes, but there was a decrease in prevalence over time in all cases except for the anal route. Variables such as temperature, dose, and the status of the donor fish appear to be more important than the selected route. Among the routes tested, anal intubation seemed to be the most promising, as it was sustained over a longer period of time, but none of them was able to reproduce the same clinical signs of infection observed in natural infections.

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